For glutamic acid fermentation system using molasses, on-line estimation of cell growth during the exponential growth phase was investigated based on the measurement of carbon dioxide evolution rate (CER). The CER was continuously calculated using microcomputer together with O2 and CO2 analyzers. It was found that a linear relationship existed between CER and cell concentration. Therefore it was possible to determine specific growth rate and specific CER accurately by measuring CER with time.
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